Effects of Liuwei Dihuang Decoction on Expressions of HIF-1α, CollⅠ, and CollⅢin Renal Interstitial Fibrosis Rats
10.3969/j.issn.1005-5304.2015.019
- VernacularTitle:六味地黄汤对肾间质纤维化大鼠肾组织中缺氧诱导因子-1α和Ⅰ、Ⅲ型胶原表达的影响
- Author:
Qun TANG
;
Xi ZHANG
;
Jiushi LEI
;
Hua WU
;
Lanlan BU
- Publication Type:Journal Article
- Keywords:
chronic kidney disease;
hypoxia-inducible factor 1α;
type I collagen;
type III collagen;
Liuwei Dihuang Decoction;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(4):67-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the mechanism of Liuwei Dihuang Decoction against renal interstitial fibrosis. Methods The rat models of renal interstitial fibrosis were induced by 5/6 nephrectomy, and were randomly divided into sham-operation group, model group, Liuwei Dihuang Decoction group and enalapril group. Liuwei Dihuang Decoction group was given 6.25 g/(kg?d) of Liuwei Dihuang Decoction for gavage, enalapril group was given 10 mg/(kg?d) enalapril for gavage, sham-operation group and model group were given 10 mL/(kg?d) of distilled water for gavage, 1 time per day for 12 weeks. Expressions of HIF-1α, CollI, and CollIII in kidney tissue were detected by immunohistochemistry. Results Compared with the sham-operation group, the expressions of HIF-1α, CollI, and CollIII of renal tissue in the model group significantly increased (P<0.05). Compared with the model group, Liuwei Dihuang Decoction group and enalapril group can inhibit the expressions of HIF-1α, CollI, and CollIII (P<0.05), and Liuwei Dihuang Decoction group was better than enalapril group (P<0.05). Conclusion The mechanism of Liuwei Dihuang Decoction against renal interstitial fibrosis may improve the renal tissue of chronic hypoxia to play the role of preventing renal interstitial fibrosis by decreasing the expressions of HIF-1α, CollI, and CollIII.
