Study on Determination Method of Luteolin-7-O-glucoside in Compound Luobuma Granule

VernacularTitle:复方罗布麻颗粒中木犀草苷含量测定方法研究
Author: Lin WU ; Fei HUANG ; Guang YANG
Publication Type:Journal Article
Keywords: Compound Luobuma Granule; luteolin-7-O-glucoside; HPLC
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;(4):91-93
CountryChina
Language:Chinese
Abstract: Objective To establish determination method of luteolin-7-O-glucoside in Compound Luobuma Granule. Methods Phenomenex luna C18 column (4.6 mm×150 mm, 5 μm) was used;aceto-0.5% acetic acid (14∶86) was set as the mobile phase at flow rate of 0.8 mL/min;the detection wavelength was 348 nm. Results The linear range of luteolin-7-O-glucoside was in the range of 0.031 9-0.796 3 μg (r=0.999 6), and the average recovery was 100.85% (n=6). Conclusion The method is specific, simple, and can improve quality standard and increase the controllability of Compound Luobuma Granule.