Study on Quality Standard of Jiangzhi Fugan Capules
10.3969/j.issn.1005-5304.2015.04.026
- VernacularTitle:降脂复肝胶囊质量标准研究
- Author:
Caidong LI
;
Xinbo PAN
;
Wei ZHANG
;
Xin WANG
- Publication Type:Journal Article
- Keywords:
Jiangzhi Fugan Capules;
quality standard;
TLC;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(4):94-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality standard of Jiangzhi Fugan Capules. Methods TLC was used to the qualitative identification of Paeoniae Radix Rubra and Salviae Miltiorrhizae Radix et Rhizoma. The contents of Paeoniflorin and Tanshinone ⅡA were determined by HPLC. The HPLC separation was performed on Phenomenex C18 column (4.6 mm×250 mm, 5 μm), with methanol-0.1% phsophonic acid (31∶69, 75∶25) as mobile phase, and the flow rate was 1.0 mL/min. Results The results of TLC showed that relevant spots were clear without interference against the negative sample. The calibration curves for Paeoniflorin and Tanshinone ⅡA were found to be liner within the range of 0.448-4.48μg, 0.057 6-0.576μg, respectively. The correlation coefficients were 0.999 4 and 0.999 5, respectively. The average recoveries were 99.93%and 99.75%, with RSD of 1.98%(n=9) and 1.70%(n=9), respectively. Conclusion The method is accurate, reliable, stable, rapid, and reproducible, and can be used for the quality control and evaluation of Jiangzhi Fugan Capules.
