The effects of E. coli ppk1 deletion in mice bladders colonization and inflammation induction
10.3969/j.issn.1006-5725.2015.05.002
- VernacularTitle:ppk1缺失对大肠埃希菌在小鼠膀胱定植及炎症诱导能力的影响
- Author:
Jiayun PAN
;
Liang PENG
;
Cong DENG
;
Xiaoyan DENG
;
Xiaoman WU
- Publication Type:Journal Article
- Keywords:
Urinary tract infection;
Escherichia coli;
ppk1
- From:
The Journal of Practical Medicine
2015;(5):697-700
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of ppk1 gene in E.coli CFT073 strain during urinary tract infection (UTI). Methods C57BL/6 mouse models of acute UTI with the wild-type(CFT073) and ppk1 mutant (△pk1) infected, were used to compare the bacteria colonization and inflammation induction abilities of bladder tissues. Results In the mouse models, the △pk1 strain showed a significantly lower infection rate (73.3% vs 93.3%) and lower adhesion frequency of bladder (0.01%vs 0.5%) than those of the CFT073 strain. The expression of IL-6 and TNF-αwere reduced in the bladder of △pk1 infected group (P<0.05). Hematoxylin-Eosin tissue staining showed that the damage degree of bladders in △pk1 infected mice were less serious than the CFT073 infected mice. Conclusion ppk1 gene plays an important role in E.coli colonization to bladder and the inflammation induction ability.
