murine colonic adenocarcinoma cell line CT26.WT
10.3969/j.issn.1006-5725.2015.05.004
- VernacularTitle:鼠源性结肠癌细胞CT26． WT对树突状细胞抗原递呈的细胞免疫抑制
- Author:
Na FENG
;
Na ZHOU
;
Yongjian DENG
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Lymphocyte;
DC cell;
Antigen presentation
- From:
The Journal of Practical Medicine
2015;(5):704-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the antigen presentation of CT26.WT via intra-peritoneal injection. Methods The intra-peritoneal injection model was made via injecting cell suspensions in mice. The spleen was isolated from BALB/c mice toco-culture with CT26.WT to detect tumor-killed ability. Phenotype identification methods and CCK8 massy were used to measure the ability of antigen presentation and stimulate T lymphocyte proliferation. IHC was used to detect the expression of B7H4 in normal and tumor tissues. Results Along with the extension of intra-peritoneal injection, the surviving number of cells was increased, contrary to the apoptosis. DC cells failed in maturation and impaired in stimulating T lymphocyte proliferation. B7H4 was higher in tumor tissues. Conclusions With the extension of intra-peritoneal injection, the mature DC cells were scared in number, resulting in the impairement of antigen-presentation. Moreover, the higher B7H4 expression in tumor tissues led to the lack of second signals which may stimulate T cells. Consequently, the ability of T cells in killing tumor cells was decreased so that they escape immunosurveillance.
