The characteristics of the genes mutations in rifampin and isoniazid resistant Mycobacterium tuberculosis clinical isolates from Baise district, Guangxi autonomous region
10.3969/j.issn.1006-5725.2015.05.012
- VernacularTitle:广西百色地区结核分枝杆菌利福平和异烟肼耐药基因特征
- Author:
Hongyu WEI
;
Xinying LONG
;
Jun LING
;
Zhenfeng XIE
;
Huaying TANG
;
Xiaofeng HUANG
;
Liandeng WEI
;
Yanchun QING
;
Yi ZENG
- Publication Type:Journal Article
- Keywords:
Tuberculosis;
Mycobacterium tuberculosis;
Multidrug resistance;
Isoniazid;
Rifampin;
rpoB;
KatG;
inhA
- From:
The Journal of Practical Medicine
2015;(5):731-734
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the characteristics of the rpoB, KatG and inhA genes mutations in rifampin and isoniazid resistant Mycobacterium tuberculosis (MTB) clinical isolates in Baise district, Guangxi autonomous region. Methods 128 MTB clinical strains were collected and isolated for drug susceptibility testing, and drug resistant strain DNA was subtracted for rpoB, KatG and inhA genes mutation analysis. Results 75%(27/36)isolates carried mutations in the rpoB gene,and 59.3%(16/27)isolates carried mutations in 531 sites. 44.1%(15/34) isolates carried mutations in KatG or inhA, and 66.7%(10/15) isolates appeared in KatG 315 site, with two new mutations found in KatG 279 and 427 site. In these mutation isolates, 13.3%(2/15) mutations appeared in inhA 5, 6.7%(1/15) in inhA 16, and 20%(3/15) in both katG and inhA. Conclusions The mutation of rpoB, katG and inhA genes in TB is highly correlated with its resistance to rifampin and isoniazid in Baise district, Guangxi autonomous region. The study will provide a basis for further understanding the anti-bacterium mechanism and quick diagnostic methods for drug-resistant tuberculosis.
