Detection of biofilm formation and analysis of biofilm-associated genes of methicillin-resistant Staphylococcus aureus in clinical isolates
10.3969/j.issn.1006-5725.2015.05.045
- VernacularTitle:临床分离耐甲氧西林金黄色葡萄球菌生物膜表型检测及其相关基因分析
- Author:
Weibin HUANG
;
Jing HUANG
;
Jiaxin ZHU
;
Hailing YANG
;
Wenbin WU
- Publication Type:Journal Article
- Keywords:
Drug Resistance;
Staphylococcus Aureus;
Biofilm;
Gene;
fnb
- From:
The Journal of Practical Medicine
2015;(5):830-833
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect biofilm formation and biofilm-associated genes of methicillin-resistant Staphylococcus aureus (MRSA) in clinical isolates. Methods The biofilm were determined by microtiter plate assay (MPA) and congo red agar (CRA) and the biofilm-associated genes icaA,sarA,fnbA,fnbB were detected by PCR in 33 strains of MRSA in clinical isolates. Results Of the 33 MRSA isolates, 29(87.9%) were MPA positive, 16(48.5%) were CRA positive; The icaA gene was present in 39.4% of isolated strains. Furthermore, 69.7% of strains harboured the sarA gene, 39.4% were fnbA positive and 75.8% were fnbB positive. As many as 87.9% strains had the ability to form biofilm in vitro. 44.8% of MRSA formed biofilm in ica-dependent mechanism and 55.2% of MRSA isolates formed biofilm in ica-independent mechanism. Of the biofilm positive MRSA, 75.9% were sarA positive, 37.9% were fnbA positive and 79.3% were fnbB positive. Conclusion Most of the MRSA strains formed biofilm in ica-independent mechanism. fnbB and sarA gene shows higher frequency among the biofilm-associated genes of MRSA, it may infer that most of the MRSA strains biofilm formation are fnbB-mediated. Meanwhile, sarA may be a positive regulator of fnbB, and thus drives the biofilm formation.
