Effects of Wugong Baidu Drink Drug-containing Serum on Expressions of Genes Related to the Apoptosis of HaCaT Cell in Psoriasis
10.3969/j.issn.1005-5304.2015.016
- VernacularTitle:蜈蚣败毒饮药物血清对银屑病HaCaT细胞凋亡相关基因表达的影响
- Author:
Suqing YANG
;
Chang LIU
;
Hailong ZHANG
;
Yuepeng AN
- Publication Type:Journal Article
- Keywords:
psoriasis;
Wugong Baidu Drink;
HaCaT cell;
inhibiting factor of apoptosis;
nuclear factor
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(4):55-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss effects of Wugong Baidu Drink on expressions of Bcl-2, NF-κB mRNA genes related to the apoptosis of HaCaT in psoriasis. Methods Wistar rats were randomly divided into 6 groups:blank group, Western medicine control group, TCM control group, and Wugong Baidu Drink high, medium, and low dose groups. They were given normal saline, methotrexate suspension, compound natural indigo capsule suspension, and the different doses of Wugong Baidu Drink for gavage, respectively. Drug-containing serum of each group was prepared, and was put in the HaCaT respectively to subculture. The intervention effect of different Drug-containing serums on expressions of objective genes were detected through PR-PCR method. Results Drug-containing serum of each group could decrease the expressions of Bcl-2 mRNA and NF-κB mRNA. The most obvious effects were shown in the groups of Western medicine control group and Wugong Baidu Drink high dose group. All Wugong Baidu Drink groups showed a dose-response relationship. Conclusion Wugong Baidu Drink probably can promote the apoptosis of HaCaT by reducing the gene expressions of Bcl-2 and NF-κB. It can also decrease immune response by regulating serum factors related to T cells to play the role of anti-psoriasis.
