Metabonomics Study on Blood of Rats with Xiaoqinglong Decoction Syndrome Based on GC-MS
10.3969/j.issn.1005-5304.2015.04.023
- VernacularTitle:基于气相色谱-质谱技术的小青龙汤证大鼠血液代谢组学研究
- Author:
Xiaohua ZHAO
;
Liqing HE
;
Jianghua GAI
- Publication Type:Journal Article
- Keywords:
GC-MS;
blood;
Xiaoqinglong Decoction Syndrome;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(4):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the endogenous metabolites in blood of rats with Xiaoqinglong Decoction Syndrome by using GC-MS;To provide evidence for researches on the essence of Xiaoqinglong Decoction Syndrome. Methods Totally 36 male Wistar rats were randomly divided into blank control group (10 rats), model group (13 rats), and Xiaoqinglong Decoction group (13 rats). Model group and Xiaoqinglong Decoction group were used to make the models of exogenous cold and internal fluid syndrome (Xiaoqinglong Decoction Syndrome). Rats in Xiaoqinglong Decoction group received gavage with Xiaoqinglong Decoction, while rats in blank control group received nothing. Blood of rats in the three groups was taken from the abdominal aorta and detected by GC-MS 15 days later, and analyzed by multivariate statistical analysis with PLS-DA. Results 13 kinds of biomarkers were found:lactic acid, 3-hydroxy-butyric acid, glycine, serine, lysine, dextrose, arachidonic acid and other metabolites. Conclusion Expressions of biomarkers, such as lactic acid and glycine, are energy metabolism, immune function, and inflammation, which provide material basis for researches on the essence of Xiaoqinglong Decoction Syndrome.
