Araloside inhibited proliferation and promotes apoptosis of HeLa cells via suppression of Akt/NF-κB pathway
10.3969/j.issn.1671-8348.2015.07.003
- VernacularTitle:楤木皂苷通过降低 Akt／NF-κB 通路抑制HeLa 细胞的增殖并促进其凋亡
- Author:
Haixia DUAN
;
Donghong LI
;
Lei BAO
;
Huan LI
;
Hongmin GAO
- Publication Type:Journal Article
- Keywords:
araloside;
HeLa cells;
Akt/NF-кB;
proliferation;
apoptosis
- From:
Chongqing Medicine
2015;(7):873-875
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of araloside on the proliferation and apoptosis of HeLa cells.Methods Human cervical cancer cell line HeLa was cultured in vitro,the experiment was divided into 4 groups as follows:blank group,araloside trea-ted groups(50,100,200 μg/mL).Normal saline and araloside (50,100,200 μg/mL)were gave,respectively.24,48 and 72 hours lat-er,the cells were collected.Cell proliferation was detected by MTT,cell apoptosis was determined by flow cytometry,the expression of pAkt1,pIкBα,NF-κB (p65),Bcl-2 and Caspase-3 were evaluated by western blot.Results Araloside obviously inhibited the pro-liferation and increased the apoptosis level of HeLa cells in a time-dose dependent manner.Moreover,Araloside significantly de-creased the phosphorylation of Akt1 and IκBα,reduced the expression of NF-κB(p65)and Bcl-2,whereas obviously increased Caspase-3 content.Conclusion Araloside could inhibit the proliferation and promote the apoptosis of HeLa cells via suppressing Akt/NF-кB signaling pathway.
