Experimental efficacy of cord blood cytokine-induced kill cells on the anti-tumor effects against bladder transitional cell
10.3760/cma.j.issn.1008-6315.2015.03.003
- VernacularTitle:脐带血细胞因子诱导杀伤细胞对人膀胱移行癌细胞体外杀伤效应的实验研究
- Author:
Feng TIAN
;
Laikun TANG
;
Chunhui WANG
- Publication Type:Journal Article
- Keywords:
Cytokine-induced kill cells of the cord blood;
Cell culture;
T24
- From:
Clinical Medicine of China
2015;(3):200-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cytokine-induced kill cells( CIK)of the cord blood on bladder transitional cell carcinoma T24 in vitro. Methods The cord blood mononuclear cells( CBMC)were induced with different cytokines such as Interleukin 2( IL-2 ),interferon γ( IFN-γ),CD3Mc-Ab into CIK cells. The proliferation ability of CIK cells was measured by calculation in different culturing time,and the phenotype of CIK cells was analyzed by the flow cytometry. Bladder transitional cell carcinoma T24 cells were mixed with CIK cells to detect the mortality of the cancer cells with CCK-8 method in vitro. Results With the cultured time of CIK cells prolong,both the number and killing activity increased. the ratio of CIK cells and T24 cells were 1:2. 5,1:5,1:10 and 1:20 when the kill rate of T24 cells by CIK cells were(18. 98 ± 2. 61)%, (35. 59 ± 4. 74)%,(45. 34 ± 5. 03)% and(69. 24 ± 5. 19)%. Conclusion The CIK cells of the cord blood is proved to be with strong anti-cancer activity against T24 cells in vitro.
