Establishment of TaqMan RT-PCR assay for Flanders virus
10.3969/cjz.j.issn.1002-2694.2015.03.005
- VernacularTitle:Flanders 病毒 TaqMan RT-PCR 检测方法的建立
- Author:
Hao LI
;
Xiaoxia HE
;
Yuxi CAO
;
Kai NIE
;
Yan LIU
;
Ying HE
;
Xiaoyan GAO
;
Shihong FU
;
Huanyu WANG
- Publication Type:Journal Article
- Keywords:
Flanders virus;
TaqMan RT-PCR;
Molecular detection
- From:
Chinese Journal of Zoonoses
2015;(3):212-215
- CountryChina
- Language:Chinese
-
Abstract:
The Flanders virus (FLAV) is a number of family Rhabdoviridae ,contains a single‐stranded ,negative‐sense vi‐ral RNA .Here we describe a molecular detection method developed for fast measurement of FLAV based on Taqman RT‐PCR method .In this study ,FLAV specific primers and probe were designed based on the FLAV L gene sequences published in GeneBank .Quantitative standard curve of FLAV TaqMan PCR was also successfully established .The specificity and stability test showed that the system is specific and the coefficient variables were all less than 1 .7% .Quantitative standard curve based on the genomic copy was drawn ,and the lowest detectable limit (LOD) of system was 100 copies/PCR ,with higher sensitivity and stability than that of the conventional RT‐PCR assay targeting the same gene .
