KPC carbapenemases among Enterobacteriaceae with carbapenem non-susceptibility
10.3969/cjz.j.issn.1002-2694.2015.03.013
- VernacularTitle:碳青霉烯酶药物不敏感的肠杆科细菌中KPC 酶的检测及分析
- Author:
Hui HUANG
;
Shumin SHE
;
Ximei ZHAN
;
Duorong WU
- Publication Type:Journal Article
- Keywords:
carbapenemase;
K lebsiella pneumoniae carbapenemases;
Enterobacteriaceae;
Modified Hodge Test
- From:
Chinese Journal of Zoonoses
2015;(3):247-250
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the prevalence and gene types of KPC in Enterobacteriaceae strains isolated from 4 tertiary general hospitals in Hainan area ,a total 43 isolates which were resistant or intermediate to imipenem or ertapenem were collected from sterile sites between August 2012 and June 2013 from 4 tertiary general hospitals in Hainan area .Modified Hodge Tests (M HT ) were performed for KPC phenotype screening .PCR amplification and DNA sequence were performed to analyze the encoding genes of KPC .Results showed that in the 43 isolates ,21 strains were positive in M HT .PCR and DNA sequence analysis confirmed that 3 isolates produced KPC‐2 .It's suggested that there were the Enterobacteriaceae carrying KPC in Hain‐an area .The encoding genes were KPC‐2 .The KPC gene could be horizontally transmitted by plasmid among different groups of bacteria .It is important to control the transmission of these Enterobacteriaceae carrying KPC .
