The effect of two different odontoblast inducer on the proliferation and apoptosis of rat adiposed-derived stem cells
10.3969/j.issn.1671-8348.2015.07.006
- VernacularTitle:2种不同成牙本质诱导液对大鼠脂肪干细胞增殖凋亡的影响
- Author:
Juanjuan WU
;
Fang TIAN
;
Qi SONG
;
Ping LI
;
Caixia ZHAO
;
Dan WEI
- Publication Type:Journal Article
- Keywords:
adiposed-derived stem cells;
tooth germ cell conditioned medium;
dentin non-collagenous protein medium;
prolif-eration;
apotosis
- From:
Chongqing Medicine
2015;(7):881-884
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of two different odontoblast inducer on the proliferation and apoptosis of rat adiposed-derived stem cells.Methods Adiposed-derived stem cells were collected by enzyme digestion from inguinal fat pads of 4 days post natal mice.Immunocytochemistry was performed to identify the cells.MTT and flow cytometry were tested the prolifera-tion and apotosis of adiposed-derived stem cells by co-cultured with tooth germ cell conditioned medium(TGC-CM)or dentin non-collagenous protein medium (DNCPM).Results Cells displayed a fibroblast-like appearance and positively expressed CD44 and CD105 when cufured to the secend yeneration.After 3 day the cells polarity changed by co-cultured.Count of cells were no obvious change by TGC-CM co-cultured,while that ruduced significantly by DNCPM co-cultured.It confirmed that the proliferation rate of ADSCs in TGC-CM group and control group is higher than DNCPM group(P <0.05),while the apotosis of that in DNCPM group is higher TGC-CM group(P <0.05).Compared with control group,the results had no significant difference(P >0.05).Conclusion TGC-CM may have more advantage as inducer in rat adiposed-derived stem cells differentiate into dentin like cells than DNCPM.
