The experimental research of nano-hydroxyapatite/polyamide 66/platelet-rich plasma compound repair of rabbit femoral bone defect
10.3969/j.issn.1671-8348.2015.07.007
- VernacularTitle:纳米羟基磷灰石／聚酰胺66／富血小板血浆复合物修复兔股骨中段骨缺损的实验研究
- Author:
Minpeng LU
;
Qunbo WANG
;
Jing DONG
;
Chunfeng CAO
;
Hao QIU
;
Shuitao ZHU
;
Zuozhong LIU
;
Chunyan JIAO
- Publication Type:Journal Article
- Keywords:
bone defect;
platelet-rich plasma;
nano-hydroxyapatite;
polyamide 66
- From:
Chongqing Medicine
2015;(7):885-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects of nano-hydroxyapatite/polyamide 66/platelet-rich plasma com-pound(n-HA/PA66/PRP)on the recovery of rabbit femur bone defect.Methods 40 New Zealand rabbits were artificially made to be bone defect by resecting the 1 cm substantia ossea with periosteum of femur,and were divided into two groups averagely depen-ding on implanted materials:experimental group(n-HA/PA66/PRP),control group (n-HA/PA66).Every five rabbits were sacri-ficed on week 2,4,8,12,and the femur healing status was observed by X ray,histology,and immunohistochemistry.Results No rabbit was infected or died,no implantation objects dropped.Gross observation,X-ray result and histology results demonstrated that the experimental group began to have a new bone tissue at 2 weeks after the operation,with the extension of time,the experimental group new bone growth speed and the quantity was better than the control group.The Lane-Sandhu method X-ray score showed that the experimental group (6.80±2.05)points and the control group(4.20±1.30)points at 12 weeks after the operation,and there were significant differences between two groups(P <0.05).Immunohistochemistry indicated that expression intensity of Vas-cular endothelial growth factor at 2 weeks and 4 weeks were significant differences between two groups(P <0.05),but there was no significant difference at 8 weeks and 12 weeks (P >0.05).Conclusion PRP combined with n-HA/PA66 artificial bone could accel-erate the healing of bone defect,and the effect of repair of bone defect is better than that of n-HA/PA66 artificial bone in the early.
