Clinical application of PCR and high resolution melting analysis for rapid identification of Brucella isolates

VernacularTitle:PCR-HRM 方法快速鉴定布鲁氏菌的初步应用
Author: Xueni LIANG ; Buyun CUI ; Lingling MAO ; Wei REN ; Jingbo YU ; Wencheng XUE ; Dongya MENG
Publication Type:Journal Article
Keywords: Brucella; molecular diagnosis; PCR-HRM analysis
From: Chinese Journal of Zoonoses 2015;(3):255-259
CountryChina
Language:Chinese
Abstract: The aim of this study is to develop a rapid and accurately species typing method for Brucella isolates by using High Resolution Melting (HRM ) analysis .Six pairs of primers were used according to the reference for the sequence of pur‐pose gene .Nineteen biotypes of six species Brucella standard strains were identified by PCR‐HRM analysis and this analysis was used to detect the 35 clinical isolates .Results showed Brucella amplified specific melting curves were different from con‐trasted strains with primer Bspp .The six species Brucella standard strains have own characteristic curve shape from each oth‐ers by PCR‐HRM analysis with five pairs of primers .Thirty‐five clinical isolates of Brucella have entirely consistent with PCR‐HRM curve shape with Brucella melitensis standard strains .So ,PCR‐HRM analysis methods can accurately identify Brucella strains ,especially clinical isolated Brucella melitensis ,and may be used in clinical microbiology laboratories .