miR-200c regulates migration of breast cancer cell BT549 by targeting Slug
10.3969/j.issn.1000-484X.2015.03.004
- VernacularTitle:miR-200 c对乳腺癌BT549细胞迁移及Slug表达的作用
- Author:
Liting JIA
;
Yuan TIAN
;
Ying SHI
;
Linlin ZHANG
;
Xiaoqian YANG
;
Shouhua RONG
;
Yuchao ZHANG
;
Jing LI
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Epithelial-mesenchymal transition;
Slug;
BT549;
E-cadherin;
miR-200c
- From:
Chinese Journal of Immunology
2015;(3):304-307
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect on the expression of Slug for the trasfection of miR-200c combined with the research on the ability of migration of breast cancer cell BT549.Methods:Chemically synthesized miR-200c mimic was trasfected into BT549 cells,which have high metastatic potential.The effect on the ability of migration of breast cancer cell BT549 for the transfection of miR-200c was analyzed by Transwell migration assay and Wound healing assay.The expression of Slug and E-cadherin mRNA was detected by Real-time PCR.The expression of Slug protein was detected by Western blot.Results:Transfection with miR-200c mimic significantly down-regulated the expression of Slug as compared with the control group (P<0.05).BT549 cell tranfected with miR-200c mimic had lower levels of migration capacity than cells in the control group (P<0.05).Conclusion:miR-200c inhibits Epithelial-mes-enchymal transition by suppressing Slug leading to down-regulation of migration capacity of breast cancer cell BT549.
