Effects of obesity induced by high-fat diet on T lymphocyte subsets in adipose tissue in mice
10.3969/j.issn.1000-484X.2015.03.006
- VernacularTitle:高脂饮食对小鼠内脏脂肪组织T细胞亚群的影响
- Author:
Qianlei ZOU
;
Guojun ZHANG
;
Yajing ZHENG
;
Huijie ZHANG
;
Ruifang WANG
;
Xia WANG
;
Xiangfeng SONG
;
Hui WANG
- Publication Type:Journal Article
- Keywords:
High-fat diet;
Blood lipid;
Thymus index;
Spleen index;
T lymphocytes
- From:
Chinese Journal of Immunology
2015;(3):314-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of obesity induced by high-fat diet on T lymphocyte subsets in the adipose tissue in mice.Methods:C57BL/6 male mice were randomly divided into 2 groups, the normal control group and high-fat diet group.After feeding 16 weeks, serum was separated and CHOL, TG, HDL, LDL and glucose levels were measured by automatic biochemical analyzer.The concentrations of TNF-αwere determined by ELISA kit.FACS was used to analyze the number of T cells and the percentage of subgroup in epididymal fat adipose tissue.Results:Compared with control group,body weight,weight gain,epididymal fat pad weight,perirenal fat weight,blood lipids,glucose and TNF-αwere significantly increased in high-fat diet group,but there were no difference in the thymus index and spleen index between the two groups.Compared with the control group,the mice fed a high-fat diet had increasing proportion of CD3+T cells,CD4+T cells and CD8+T cells in adipose tissue and there was a significant increase on the proportion of Th1 and Th17 sublineage in the HFD group.Conclusion:High-fat diet induced obesity can lead to the increasing proportion of CD3+T cells,CD4+T cells and CD8+T cells in epididymal fat pads and generate a progressive Th1 and Th17 bias.
