Sesamin attenuates inflammation response in a murine model of asthma
10.3969/j.issn.1001-1978.2015.03.023
- VernacularTitle:芝麻素抗哮喘炎症作用研究
- Author:
Liangchang LI
;
Hongmei PIAO
;
Guanghai YAN
;
Xiangzheng QIN
;
Guangzhao LI
- Publication Type:Journal Article
- Keywords:
asthma;
sesamin;
airway inflammation;
nu-clear factor-kappa B;
interleukin-4;
interleukin-5;
inter-feron-γ
- From:
Chinese Pharmacological Bulletin
2015;(3):411-415
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of sesamin on inflammation response of asthma and to explore its possible mechanism of action. Methods Forty male BALB/c mice were randomly divided into five groups with 8 mice in each group: normal group, ovalbumin ( OVA) group, sesamin low dose group, sesamin high dose group and dexamethasone( DXM) group. Asthma model mice were induced by OVA in vivo. The left lung was isolated for pathological examination. Experi-ment of ELISA and Western blot were used to deter-mine the effect of sesamin on IL-4 , IL-5 , IL-13 and IFN-γ expression. Hematoxylin and eosin stain was used to investigate pathological examination in lung tis-sue. Western blot was performed to detect the IκBαphosphorylation and NF-κB nuclear translocation. Re-sults The mice developed the following pathophysio-logical features of asthma: increased numbers of in-flammatory cells, increased levels of IL-4, IL-5 and IL-13 , decreased level of IFN-γ in bronchoalveolar lavage fluids ( BALF ) and lung tissues ( P <0. 05 ) , and increased IκBα phosphorylation and NF-κB nucle-ar translocation in lung tissues ( P <0. 05 ) . Adminis-tration of sesamin markedly reduced airway inflammato-ry cell recruitment, reduced the production of IL-4, IL-5 , IL-13 and increased IFN-γ in BALF and lung tissues( P <0. 05 ) . The increased IκBα phosphoryla-tion and NF-κB nuclear translocation after OVA inhala-tion were inhibited by the administration of sesamin. Conclusion Sesamin attenuates inflammation re-sponse of asthma through suppression of NF-κB activa-tion.
