Simultaneous Determination of Five Active Components in Sijiganmao Tablets by HPLC
- VernacularTitle:HPLC法同时测定四季感冒片中5种有效成分的含量
- Author:
Zhaoxian ZHENG
;
Dantong ZHAO
;
Peng ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Multicomponent determination;
Sijiganmao tablets;
5-O-Methylvisammioside;
Prim-O-glucosycimifugin;
Hesperidin;
Rosmarinic acid;
Forsythin
- From:
China Pharmacist
2015;(3):487-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an HPLC method for the simultaneous determination of five active components ( 5-O-methyl-visammioside, prim-O-glucosycimifugin, hesperidin, rosmarinic acid and forsythin) in Sijiganmao tablets. Methods:The samples were separated on an Agilent C18 column (250 mm × 4. 6 mm,5 μm) by acetonitrile-0. 1% formic acid solution with gradient elution. The detection wavelength was 283nm,the flow rate was 1. 0μl·min-1 ,the column temperature was 30℃,the detection wavelength was 283 nm,and the sample size was 10μl. Results:The complete separation was obtained for the five active compounds. The five regression e-quations showed good linear relationships. The average recoveries of the compounds were between 95. 0% and 105. 0%. Conclusion:The established method is accurate, reliable, simple and effective, which can be used in the quality control of Sijiganmao tablets.
