miR-708-5p accelerates migration of human mesenchymal stem cells by repressing TMEM88 expression
10.3969/j.issn.1000-4718.2015.02.009
- VernacularTitle:miR-708-5 p 通过抑制 TMEM88表达促进人骨髓间充质干细胞迁移
- Author:
Jiao LI
;
Ningning LIU
;
Ming HU
;
Bohang ZENG
;
Jun DONG
;
Shiming LIU
- Publication Type:Journal Article
- Keywords:
Human mesenchymal stem cells;
MicroRNA-708-5p;
Age;
Cell migration
- From:
Chinese Journal of Pathophysiology
2015;(2):239-244
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of microRNA-708-5p (miR-708-5p) on the migration of human mesenchymal stem cells (hMSCs).METHODS:The expression of miR-708-5p was determined by miRNA arrays and re-al-time PCR.By transfection of miR-708-5p mimic or inhibitor, the up-regulation or down-regulation of miR-708-5p ex-pression in hMSCs was evaluated .The cell scratch and Transwell tests were used to detect the migration capability of hM-SCs.The effects of transmembrane protein 88 (TMEM88), a miR-708-5p target gene, onβ-catenin expression and migra-tion of hMSCs were detected .RESULTS:The expression of miR-708-5p was down-regulated in the old hMSCs compared with the young hMSCs.Up-regulation of miR-708-5p resulted in increasing migration of hMSCs.Conversely, down-regula-tion of miR-708-5p resulted in decreasing cell migration .The expression of TMEM88 was up-regulated in the old hMSCs compared with the young hMSCs , while the expression of β-catenin was down-regulated.Directly repression of TMEM88 expression increased the β-catenin expression and migration of hMSCs .The regulation of miR-708-5p on hMSCs was atten-uated by inhibiting the expression of miR-708-5p and TMEM88 together.CONCLUSION:miR-708-5p increases β-cate-nin expression and Wnt/β-catenin activity by repressing TMEM 88, thus enhancing the migration of hMSCs .
