Effect of ribosomal protein S3 Ser209 on NF-κB transcriptional activity and its DNA binding capacity

VernacularTitle:核糖体蛋白RPS3 Ser209影响NF-κB转录活性及其与DNA结合能力
Author: Hengxi GUO ; Hui CHEN ; Yiqun ZHAN ; Miao YU ; Xiaoming YANG
Publication Type:Journal Article
Keywords: RPS3; NF-κB; transcription activity; DNA-binding
From: Military Medical Sciences 2015;(2):117-122
CountryChina
Language:Chinese
Abstract: Objective To construct expression vectors of human ribosomal protein S 3(RPS3) and RPS3Ser209 mutant in orcler to investigate the effect of RPS3Ser209 mutant on NF-κB signaling pathway and DNA binding capacity .Methods The vector RPS3-myc was amplified by polymerase chain reaction ( PCR) from the human liver cDNA and subcloned into pcDNA-3.1myc-HisB.RPS3S209A represented mutant RPS3 expression vectors, in which the designated amino acid was mutated to an alanine residue .Dual luciferase reporter gene assay was used to detect the NF-κB transcription activity in HEK293 cells,immunofluorescence to detect RPS3 location, and EMSA to examine NF-κB DNA-binding activity.Results The expression vectors of RPS3-myc and RPS3S209A-myc were constructed.Compared with wild-type RPS3,the nucleus translocation, transactivation activity of NF-κB and DNA binding ability of RPS3S290A were reduced significantly .Conclu-sion The impact of RPS3 on NF-κB signaling pathway depend on its serine 209.