Development of up-converting phosphor technology based lateral flow assay for quan-titative detection of foodborne pathogens
10.7644/j.issn.1674-9960.2015.02.012
- VernacularTitle:基于上转发光免疫层析技术的常见食源性致病菌快速检测方法研究与评价
- Author:
Chunfeng LI
;
Yong ZHAO
;
Xiaoying WANG
;
Pingping ZHANG
;
Xiao LIU
;
Chongyun SUN
;
Ruifu YANG
;
Chengbin WANG
;
Lei ZHOU
- Publication Type:Journal Article
- Keywords:
up-converting phosphor nano-particles;
lateral flow;
quantitative detection;
food-borne pathogen;
Salmo-nella paratyphi A;
Salmonella paratyphi B;
Escherichia coli O157∶H7;
Vibrio parahaemolyticus
- From:
Military Medical Sciences
2015;(2):128-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an up-converting phosphor technology based lateral flow (UPT-LF) assay for rapid detection of Salmonella paratyphi A, S.paratyphi B, Escherichia coli O157 ∶H7 and Vibrio parahaemolyticus. Methods With up-converting phosphor nano-particles ( UCP-NPs ) as the bio-marker, four double-antibody-sandwich mode based UPT-LF strips for detecting the above mentioned four pathogens were prepared respectively and their sensitivi-ty, accuracy, linearity and specificity were evaluated .Furthermore, the feasibility of detecting bacteria in food samples was evaluated by different food samples artificially contaminated with less than 10 CFU target pathogens .Results The sensitivi-ty of UPT-LF assays for four pathogens was 105 ~106 CFU/ml with excellent specificity .The four strips had a good linear response with the linear fitting coefficient of determination (r) for each target pathogen ranging from 0.985 to 0.996.The positive rate of detecting pathogens from samples was acceptable .Conclusion The four developed UPT-LF strips provide a new choice for rapid , specific and sensitive and quantitative detection of S.paratyphi A , S.paratyphi B, E.coli O157∶H7 and V.parahemolyticus.
