Protocol of automatically processing 2 U frozen red blood cells
10.7644/j.issn.1674-9960.2015.02.014
- VernacularTitle:2单位冰冻红细胞自动化洗涤程序研究
- Author:
Yan WANG
;
Minxia LIU
;
Hai CHENG
;
Wenjing LI
;
Jiexi WANG
;
Wei DU
;
Ying HAN
;
Donggen WANG
;
Xuebing JIANG
- Publication Type:Journal Article
- Keywords:
frozen RBC washing machine,dialysis type;
2 U of frozen red blood cells;
washing protocol;
cryopreserva-tion
- From:
Military Medical Sciences
2015;(2):136-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the processing protocol of the frozen erythrocyte ( RBC) washing machine of the dialysis type to enhance its efficiency of treating the 2 U frozen RBCs .Methods The frozen RBC washing machine for dialysis was used to deglycerolize the 2 U frozen RBCs after thawing .Measures including adjustment of the Pump speed , washing buffer adding and solution osmolarity were taken to decrease the processing time .Hemoglobin(Hb) content, Hb recovery, residual glycerol, free Hb content, deforming ability, and apoptosis of the deglycerolized RBCs were assessed to evaluate the efficiency of the improved protocol .Results As for the cells processed respectively by the previous and improved protocols, contents of Hb were (35.03 ±4.20) g and (53.82 ±2.08) g, respectively; Hb recoveries were (61.10 ±8.46)% and (80.22 ±3.73)%,respectively.Osmolarities representing residual glycerol were (307.00 ± 14.50)mOsm and (322.00 ±29.00)mOsm.Free Hb values were (0.74 ±0.04) and (0.80 ±0.08)g/L.The processing time was (1.64 ±0.31) and (1.04 ±0.16) h,respectively.There were significant differences in Hb content , Hb recovery, and deforming ability between the cells processed by the two protocols respectively .However , there were no significant changes in glycerol contents , free Hb, and apoptosis extent between the cells processed by the two protocols .Conclusion The innovated protocol can improve the quality of washed frozen RBCs , decrease the processing time and enhance the efficiency of the machine.
