Adiponectin attenuates H2 O2-induced SH-SY5 Y cell injury and tau hyper-phosphorylation via activating PP2 A
10.3969/j.issn.1000-4718.2015.02.003
- VernacularTitle:脂联素通过激活 PP2A 减轻 H2O2诱导的SH-SY5Y 细胞损伤及 tau 蛋白过度磷酸化
- Author:
Zhe SONG
;
Chao XUE
;
Xiaoman ZHANG
;
Yemin ZHANG
;
Xiaohua HE
;
Jun YIN
- Publication Type:Journal Article
- Keywords:
Adiponectin;
Hydrogen peroxide;
Protein phosphatase 2A;
Glycogen synthase kinase-3β;
Tau proteins
- From:
Chinese Journal of Pathophysiology
2015;(2):207-212
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effects of adiponectin on H 2 O2-induced cell injury and tau hyperphosphorylation in human neuroblastoma SH-SY5Y cells.METHODS:Cell viability was determined by MTT assay .H2 O2-induced cell in-jury and morphological changes in the SH-SY5Y cells with or without adiponectin treatment were observed .The level of tau phosphorylation as well as the activities of protein phosphatase 2A (PP2A) and of glycogen synthase kinase-3β(GSK-3β) were examined by Western blotting . RESULTS: Adiponectin significantly attenuated H 2 O2-induced cell injury (P<0.01).Adiponectin upregulated the activity of PP2A and decreased phosphorylation levels of tau under the stimula-tion with H2O2(P<0.01).Okadaic acid, a specific inhibitor of PP2A, blocked the protective effects of adiponectin (P<0.01).Adiponectin increased the phosphorylation level of GSK-3βat Ser9 site under H2O2stimulation (P<0.01).CON-CLUSION:Adiponectin protects SH-SY5Y cells against H2O2-induced cell injury and decreases tau hyperphosphorylation by activating PP2A and inactivating GSK-3β.
