Changes of CBS expression in brain following ischemia and reperfusion of limbs
10.3969/j.issn.1000-4718.2015.02.004
- VernacularTitle:大鼠肢体缺血-再灌注后脑 CBS 表达的变化及意义
- Author:
Yuling WANG
;
Huamin MA
;
Bing WANG
;
Yan ZHANG
;
Xinli HUANG
- Publication Type:Journal Article
- Keywords:
Brain;
Extremities;
Reperfusion injury;
Cystathionine β-synthase;
Hydrogen sulfide
- From:
Chinese Journal of Pathophysiology
2015;(2):213-218
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To detect the changes of cystathionine β-synthase (CBS) expression in the brain following ischemia-reperfusion of hind limbs in the rats and to elucidate their significance .METHODS:Hind limb ischemia was in-duced by clamping infrarenal aorta with a microvascular clip and brain injury was made by following reperfusion .The brain tissue was obtained from the animals subjected to sham operation , 4 h of ischemia without reperfusion and 3 h, 6 h, 12 h, 24 h of reperfusion following 4 h of ischemia .The expression of CBS at mRNA and protein levels was measured at different time points by reverse transcription-polymerase chain reaction ( RT-PCR) and Western blotting .The CBS activity and hy-drogen sulfide ( H2 S) concentration in the brain were determined by a universal microplate reader .The observation of path-ologic changes of the brain was made following the inhibition of CBS by hydroxylamine .RESULTS:The relative expression of CBS at mRNA and protein levels in IR group significantly increased compared with sham group .It reached a peak at 12 h (P<0.01), and returned to baseline at 24 h (P>0.05).This time course correlated with increased CBS activity and H2 S concentration .No statistically significant difference in the above indexes between sham operation group and ischemia group was observed (P>0.05).Inhibition of CBS activity induced more severe brain injury in IR group .CONCLUSION:Up-regulation of CBS/H2 S pathway is involved in the protection for neurons during the ischemia-reperfusion of hind limbs .
