Application of STR-PCR in monitoring the effect of allogeneic hematopoietic stem cell transplantation
10.3969/j.issn.1671-8348.2015.19.014
- VernacularTitle:STR-PCR在异基因造血干细胞移植中植入效果监测的应用
- Author:
Tao ZHANG
;
Fang WANG
;
Sumin ZHU
;
Wei MAO
;
Xia HUANG
- Publication Type:Journal Article
- Keywords:
hematopoietic stem cell transplantation;
STR-PCR;
effect examination
- From:
Chongqing Medicine
2015;(19):2632-2634
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish multiple short tandem repeat (STR) amplification by fluorescence labeling polymerase chain reaction (PCR) for monitoring the effect of allogeneic hematopoietic stem cell transplantation .Methods Sixty‐five patients were analyzed .DNA from peripheral blood of donors and recipients in pre transplantation and post transplantation were extracted , 15 STR loci and sexual loci were amplified by PCR .Results After allo‐HSCT ,54 patients obtained type of donors ,but 3 patients did not ;eight patients showed mixed chimerism .Two cases of type of donor converted into mixed chimerism and two cases of mixed chimerism converted into type of donors after some time .The time of earliest detection ,duration and transformation of each state was different .The earliest detection showed on the 16th day after surgery ,and the last one showed five months later .As to the du‐ration ,the shortest and longest were two months and seven months ,respectively .Conclusion The key factors that significantly in‐fluence the application of STR‐PCR in monitoring the effect of allogeneic hematopoietic stem cell transplantation were samples ,STR loci and proper monitoring time .So to establish the suitable method for this region could truly take maximum advantage of the PCR‐STR method .While a appropriate detecting time and STR loci should be chose .
