Effects of inhibiting myosin light chain kinase on endothelin-1 induced proliferation and apoptosis of pulmonary arterial smooth muscle cells in rats
10.3969/j.issn.1000-4718.2015.02.012
- VernacularTitle:抑制肌球蛋白轻链激酶活性对内皮素-1诱导的大鼠肺动脉平滑肌细胞增殖及凋亡失衡的影响
- Author:
Yongyao YANG
;
Tianhe YANG
;
Qingan JIANG
;
Long YANG
;
Feng TANG
;
Hongwen TAN
- Publication Type:Journal Article
- Keywords:
Myosin light chain kinase;
Endothelin-1;
Pulmonary artery smooth muscle cells;
Cell prolifera-tion;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(2):256-260
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of inhibiting myosin light chain kinase ( MLCK) on endothelin-1 (ET-1) induced proliferation and apoptosis of rat pulmonary artery smooth muscle cells (PASMCs).METHODS: Rat PASMCs were cultured and stimulated with ET-1.The cells were randomly divided into control group , ET-1 group and ET-1+MLCK inhibitor group (ET-1+M).Western blotting, MTT assay, [3H]-TdR incorporation and flow cytometry were employed to test the expression of myosin light chain (MLC) and MLCK, cell proliferation, cell cycle and apoptotic rate of PASMCs ,respectively .The phosphorylation of MLC was determined by glycerol-PAGE coupled with Western blotting .RE-SULTS:Compared with control group , the protein expression of MLCK and MLC phosphorylation significantly enhanced af -ter ET-1 stimulation.ET-1 markedly induced the proliferation and decreased the percentage of apoptotic rate in the PASMCs.However, pretreatment with ML-7, a MLCK inhibitor, significantly reversed the above effects induced by ET-1. CONCLUSION:MLCK inhibitor effectively inhibits the ET-1-induced proliferation and the cell cycle progression .
