Isolation and pure culture of microvascular endothelial cells from the fetal skin.
10.3349/ymj.1996.37.3.186
- Author:
Myung Soo CHA
1
;
Dong Kyun RAH
;
Kwang Hoon LEE
Author Information
1. Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Fetal skin;
microvascular endothelial cells;
isolation;
Ulex europaeus I;
magnetic beads
- MeSH:
Cells, Cultured;
Endothelium, Vascular/*cytology;
Factor VIII/analysis;
Female;
Fetus;
Human;
Intercellular Adhesion Molecule-1/analysis;
Pregnancy;
Skin/blood supply;
Support, Non-U.S. Gov't;
Vascular Cell Adhesion Molecule-1/analysis
- From:Yonsei Medical Journal
1996;37(3):186-193
- CountryRepublic of Korea
- Language:English
-
Abstract:
Microvascular endothelial cells were purely isolated from human fetal skin using magnetic particles. The principle of this technique is based on the selective binding of the lectin Ulex europaeus I (UEA I) to the endothelial cell surface via fucose residues. Initially UEA I was covalently bound to tosyl-activated magnetic polydisperse polymer particles (Dynabeads) and then the UEA I-coated beads were collected using a magnetic particle concentrator (MPC). Endothelial cells were isolated by extracting microvascular segments from trypsin-treated fetal skin tissue and were purified by sieving with nylon mesh and by 35% Percoll gradient centrifugation. For further purification, the obtained cells were incubated with UEA I-coated Dynabeads. The endothelial cells bound to the Dynabeads were collected using MPC. This is a simple and reproducible technique for isolating a pure population of microvascular endothelium from the fetal skin.
