Detection of the fusion genes by multiplex RT-PCR and its clinical significances in leukemia

VernacularTitle:多重巢式反转录-聚合酶链反应检测白血病相关融合基因及其临床意义
Author: Xiucai XU ; Chaojie HU ; Weibo ZHU ; Zhiwei WU ; Zimin SUN
Publication Type:Journal Article
Keywords: Leukemia; Chromosomal aberrations; Gene fusion; Polymerase chain reaction
From: Journal of Leukemia & Lymphoma 2009;18(12):717-720
CountryChina
Language:Chinese
Abstract: Objective To analyze the fusion genes derived from 29 types of chromosome structural aberrations in leukemia patients,and the significances on the MICM typing,risk grouping,and minimal residual disease(MRD)monitoring of leukemia.Methods The bone ulan-ow or blood samples from 141 leukemia patients were analyzed with a novel multiplex nested RT-PCR.In addition.chromosomal karyotypes were investigated in some patients.Results Of the 141 leukemic samples,66(46.8%)carried 13 types of MLL/AF6,MLL/AF9,dupMLL MLI/ENL,CBFβ/MYH11 and TLS,ERG.Fusion genes were positive in 27 of 57 ALL patients(47.4 q%),and 33 of 78 AML patients(42.3%),respectively.In these ALL or AML patients,7 or 6 chromosome structural aberrations were found. Conclusion This multiplex nested RT-PCR reaction could screen 29 types of chromosome structural aberrations at the same time. It may be helpful for the diagnosis, risk grouping,prognosis evaluation and the detection of minimal residual diseases after chemotherapy and bone marrow transplantation in these leukemia patients.