Direct Molecular Analysis of Garlic Using Internal Extractive Electrospray Ionization Mass Spectrometry
- VernacularTitle:电喷雾内部萃取电离质谱直接分析蒜瓣组织的研究
- Author:
Hua ZHANG
;
Liang ZHU
;
Huanwen CHEN
- Publication Type:Journal Article
- Keywords:
Internal extractive electrospray ionization;
Garlic;
Direct analysis;
Mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2014;(11):1634-1639
- CountryChina
- Language:Chinese
-
Abstract:
Theapplicationofinternalextractiveelectrosprayionizationmassspectrometry(iEESI-MS)was extended to direct molecular analysis of garlic tissues. By obviating time-consuming sample preparations, fragile active garlic substances such as organosulfur compounds ( e. g. , alliin, allicin ) were successfully detected and identified via collision-induced dissociation ( CID) , together with amino acids ( e. g. , arginine) and saccharides ( glucose, polysaccharides) . Mass spectral fingerprints of different kinds of garlic cloves, as well as various post-treatment ones, were further processed via principal component analysis ( PCA) to better visualize the differences. Our experimental results indicated that iEESI-MS allowed rapid recognition of metabolic changes in the garlic tissue subject to various external stimuli. The merits included simplicity of analysis, high speed ( less than 2 min per sample ) , good specificity, and minimal disturbance to the bioactivity of analytes.
