Identification of Metabolized Polychlorinated Biphenyls in Fishery Products by Gas Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.140346
- VernacularTitle:气相色谱串联质谱法用于水产品中多氯联苯二代污染物鉴别
- Author:
Yongfu SHI
;
Youqiong CAI
;
Huijuan YU
;
Liangliang TIAN
;
Xuanyun HUANG
;
Dongmei HUANG
- Publication Type:Journal Article
- Keywords:
Gaschromatography-tandemmassspectrometry;
Polychlorinatedbiphenyls;
Metabolized pollutants;
Hydroxylated polychlorinated biphenyls
- From:
Chinese Journal of Analytical Chemistry
2014;(11):1640-1645
- CountryChina
- Language:Chinese
-
Abstract:
Gaschromatography-tandemmassspectrometrywasadoptedtoidentifythehydroxylatedmetabolites of polychlorinated biphenyls and analyze the target compounds in fishery products. After the silylation derivatizaiton at 60 ℃ for 40 min, the MS spectrum of derivatives was obtained by GC/MS/MS under fullscan mode, and the fragment ions with the highest relative abundance were identified as m/z 399, 399, 399, 399, 399, 448 and 469, respectively which were defined as the precursor ion for product ion scan. The MS/MS spectrum was obtained under multiple reaction mode ( MRM ) by GC/MS/MS. And the product ions corresponded with the precursor ions were m/z 364, 364, 364, 364, 364, 433 and 434, respectively. The target compounds can be identified accurately by GC/MS/MS. In the present study, we took seven hydroxylated polychlorinated biphenyls as target compounds including 3-OH-PCB101 , 4-OH-PCB106 , 4-OH-PCB112 , 4'-OH-PCB101 , 3-OH-PCB118 , 3-OH-PCB138 and 3-OH-PCB180 . According to the identification method stated as above, the MS and MS/MS spectra of seven compounds were obtained. The compounds can be qualified and quantified by the identification method. The limits of detection ranged from 0. 02 to 0. 14 μg/L, and the limits of quantification ranged from 0. 09 to 0. 48 μg/L. The method has been applied to identify the hydroxylated polychlorinated biphenyls in fishery products.
