Depletion of Chicken Egg White Proteins by Pending Templates Imprinted Polymers
10.11895/j.issn.0253-3820.140491
- VernacularTitle:待定模板印迹聚合物对高丰度鸡蛋清蛋白质的脱除
- Author:
Chun YANG
;
Xinjie LUAN
;
Guofeng LIU
;
Xinglu ZHOU
;
Jian WANG
;
Xiaoya HU
- Publication Type:Journal Article
- Keywords:
Pendingtemplatesimprintedpolymer;
Chickeneggwhite;
Proteindepletion;
Enrichment
- From:
Chinese Journal of Analytical Chemistry
2014;(11):1651-1655
- CountryChina
- Language:Chinese
-
Abstract:
Eggwhitesolutionswereusedas“pendingtemplates”topreparemolecularimprintedpolymers. The obtained polymers acted as stationary phases, exhibiting abilities to deplete abundant proteins in the egg white. The abilities of protein depletion of the imprinted polymers could be adjusted by using different concentrations of egg white in preparing the polymers. After chromatographically processed in a home-made syringe system, abundant proteins such as ovalbumin, lysozyme, and ovotransferrin were removed from egg white sample solutions. Along with the disappearance of the mass spectrum peaks of these proteins, the signals of other components became more obvious. Meanwhile according to reference data and molecular weights, they were reasonably ascribed to be ovalbumin-related proteins, ovotransferrin-related proteins, ovomucoids or flavoproteins respectively. Conclusively, pending imprinting method has the power to deplete abundant proteins, and to retain and enrich posttranslationally modified species at the same time.
