A Study of Miriplatin and Its Pt-metabolites in Beagle Dog Plasma by Size Exclusion Chromatography-Inductively Coupled Plasma Mass Spectrometry
10.11895/j.issn.0253-3820.140545
- VernacularTitle:尺寸排阻色谱-电感耦合等离子体质谱联用研究比格犬血浆中米铂及铂代谢物形态
- Author:
Deye LIU
;
Yuanbin HAO
;
Wenru HAN
;
Jian LI
;
Zongli HUO
;
Hualiang LIU
- Publication Type:Journal Article
- Keywords:
Miriplatin;
Platinum-metabolites;
Speciation;
Pharmacokinetics
- From:
Chinese Journal of Analytical Chemistry
2014;(11):1667-1672
- CountryChina
- Language:Chinese
-
Abstract:
AstudyofPt-metabolitesfromanewanti-hepatomadrugmiriplatinwasimportanttomiriplatin's pharmacology research. Therefore, a method based on size exclusion chromatography-inductively coupled plasma mass spectrometry ( SEC-ICP-MS) was developed to study miriplatin and its Pt-metabolites in Beagle dog plasma. This method could be used to study total platinum concentration half-quantitatively. Compared with traditional ICP-MS direct determination, data acquired from this SEC-ICP-MS method were almost the same. By using BioSep-s2000 column, 25 mmol/L of pH 7. 2 phosphate buffer as eluent, and Pt-195 as detecting isotope, we discovered miriplatin with its four Pt-metabolites in dog plasma after intra-hepatic artery administration. The main Pt-metabolite was m2 , which associated with plasma proteins. Miriplatin in plasma did not bind with plasma proteins. According to calculation, the ratio of miriplatin/m2 first decreased rapidly, and then slowly increased to its second climax, finally slowly decreased.
