Difference of Apolipoprotein E promoter polymorphism distribution between Kazak and Han patients with coronary heart disease in Xinjiang
10.3969/j.issn.1000-484X.2014.11.023
- VernacularTitle:载脂蛋白E基因启动子区多态性分布在新疆哈汉民族冠心病患者之间差异性研究
- Author:
Shexue TAO
;
Jinxing LI
;
Yuyan LEI
;
Shuang LIN
- Publication Type:Journal Article
- Keywords:
Apolipoprotein E gene promoter;
Single nucleotide polymorphism;
Coronary heart disease;
Racial difference
- From:
Chinese Journal of Immunology
2014;(11):1540-1542
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the Xinjiang Kazakh,Han nationality patients with coronary heart disease apolipoprotein E gene promoter region rs405509 (G-T),rs449647 (A-T),rs7259620 (G-A) whether there exist differences in the two national distribution between loci polymorphism.Methods:201 cases were studied.DNA product was extracted by using the PheNol-chloroform method and the PCR outcome was purified.We take advantage of multiple single base extension reaction to make DNA Sequencing on The ABI3130XL.Results: The Kazak and Han patients in Xinjiang area in the apolipoprotein E gene promoter rs449647 ( A-T) was statistically significant differences in genotype and allele in two ethnic groups (P<0.05),rs405509 (G-T) loci and rs7259620 (G-A) genotype and alleles in two ethnic groups had no difference statistical significance ( P>0.05).Conclusion:Apolipoprotein E promoter rs449647 ( A-T ) genotype and allele polymorphism have significant differences between Han nationality and Kazak nationality in Xinjiang,others have no statistic difference between the two ethnic groups.
