Rapid diagnosis of trisomy 21 syndrome by denaturing high-performance liquid chromatography
10.3969/j.issn.1673-4130.2014.21.006
- VernacularTitle:变性高效液相色谱法快速诊断21三体综合征
- Author:
Xiaoli HUANG
;
Duanrong MEI
;
Yi HUANG
;
Yanan WU
;
Hao CHEN
- Publication Type:Journal Article
- Keywords:
trisomy 21 syndrome;
short tandem repeat;
denaturing high-performance liquid chromatography
- From:
International Journal of Laboratory Medicine
2014;(21):2879-2880
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the double DNA of trisomy 21 syndrome patients by denaturing high-performance liquid chro-matography(DHPLC) in order to rapidly diagnose trisomy 21 syndrome .Methods To amplify DNA fragments of three short tan-dem repeats of D21S111 ,D21S1411 and D21S1412 using corresponding primers .Then DHPLC was introduced to analyze the DNA fragments in the temperature of 50 ℃ .Results DHPLC elution profiles of D21S111 ,D21S1411 and D21S1412 of normal control showed one peak or two peaks with the same altitude .However DHPLC elution profiles of 21 trisomy syndrome patients showed two peaks of different altitudes ,which one′s altitude was twice than another .Conclusion DHPLC is a sensitive ,convenient ,auto-matic and highly-efficient method to diagnose trisomy 21 syndrome and can be widely used in the clinic diagnosis .
