Determination of Oleanolic Acid in She Medicine Radix of Aralia Chinensis L. by HPLC
- VernacularTitle:HPLC法测定畲药老虎吊中齐墩果酸的含量
- Author:
Huali YU
;
Weiying WANG
;
Juhua MAO
;
Zhangjin CHEN
- Publication Type:Journal Article
- Keywords:
Traditional She medicine;
Radix of Aralia chinensis L.;
Oleanolic acid;
HPLC
- From:
China Pharmacist
2014;(11):1963-1964
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of oleanolic acid in She medicine radix of Aralia chinen-sis L. . Methods:The HPLC analysis was performed on a Waters XBridge-C18 (250 mm × 4. 6 mm, 5 μm) column. The mobile phase was methanol-0. 1 mol·L-1 ammonium acetate solution (83∶17) and the flow rate was 1. 0 ml·min-1 . The detection wavelength was 210 nm, the column temperature was 20 ℃, and the injection volume was 10 μl. Results:Oleanolic acid in radix of Aralia chinensis L. had a good separation from the other components, a good linearity was obtained within the range of 72. 52-725. 2 μg·ml-1 ( r=1. 000 0), and the average recovery was 98. 05%(RSD=1. 89%, n=6). Conclusion:The method is simple, accurate, reproducible and applicable in the assay of oleanolic acid in radix of Aralia chinensis L. .
