Determination of Capsaicine, Dihydrocapsaicin, Imperatorin and Isoimperatorin in Guanjie Jietong Plas-ters by HPLC
- VernacularTitle:HPLC法同时测定关节解痛膏中的辣椒素、二氢辣椒素、欧前胡素和异欧前胡素
- Author:
Jun YAN
;
Li WANG
;
Zhuo JIN
- Publication Type:Journal Article
- Keywords:
Guanjie Jietong plasters;
Capsaicine;
Dihydrocapsaicin;
Imperatorin;
Isoimperatorin
- From:
China Pharmacist
2014;(11):1965-1967
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC method for the determination of capsaicine, dihydrocapsaicin, imperatorin and isoim-peratorin in Guanjie Jietong plasters. Methods:A CAPCELL PAK C18 column was used as the chromatographic column, and the flow rate was 0. 8 ml·min-1. The mobile phase A consisted of methanol-acetonitrile(1∶1), the mobile phase B was of 1% phosphoric acid. The detection wavelength was 280 nm and 254 nm, respectively. Results:There was a good linear relationship between the con-centrations and the peak areas within the range of 0. 298-5. 956μg(r=0. 9998) for capsaicine, 0. 152-3. 044μg(r=0. 999 6) for di-hydrocapsaicin, 0. 018-0. 352 μg(r=0. 999 5) for imperatorin and 0. 010-0. 204 μg(r=0. 999 3) for isoimperatorin. The average re-covery was 97. 8%(RSD=1. 02%), 97. 0%(RSD=0. 76%), 96. 6%(RSD=0. 65%) and 98. 1%(RSD=1. 35%), respectively. Conclusion:The method is convenient, accurate, sensitive and repeatable, which can be used in the determination of capsaicine, di-hydrocapsaicin, imperatorin and isoimperatorin in Guanjie Jietong plasters.