Research of the internal quality controlling method for 17-OHP determination
10.3969/j.issn.1673-4130.2014.21.035
- VernacularTitle:17-羟孕酮测定的室内质量控制方法研究
- Author:
Jing WANG
;
Hong ZHAO
;
Jinfu ZHOU
- Publication Type:Journal Article
- Keywords:
17-OHP;
time-resolved fluoroimmunoassay;
instant method;
internal quality control
- From:
International Journal of Laboratory Medicine
2014;(21):2943-2944
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find an internal quality controlling method for 17-OHP determination by time-resolved fluoroimmuno-assay .Methods 20 quality control data were collected .The data were analyzed by using L-J method ,instant method and improved instant method .Results were used for the construction of quality control charts .Result The first three quality control data had a great impact on the following judgments of internal quality controlling when instant method was used .The subsequent results might be false acceptance .Improved instant method could effectively reduce the situations of false run-away and false acceptance ,which was suitable for the internal quality control of 17-OHP determination by time-resolved fluoroimmunoassay in newborn screening . Conclusion There are many steps of manual operations in 17-OHP determination of time-resolved fluoroimmunoassay .The details of these operations have great impacts on the experimental results .Thus ,the operations of 17-OHP test should be specified and exe-cuted strictly according to requirement .
