Relationship between efflux pump phenotype and genotype in multiple drug Acinetobacter baumannii
- VernacularTitle:多重耐药鲍曼不动杆菌外排泵表型和基因型的研究
- Author:
Jingna SUN
;
Wei DONG
;
Shuai ZHAO
;
Guoxin WANG
;
Zeshi LIU
;
Zheng ZHANG
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Drug resistance,multiple,bacterial;
Carbonyl cyanide m-chlorophenyl hydrazone;
Phenotype;
Genotype
- From:
Chinese Journal of Laboratory Medicine
2014;(10):763-766
- CountryChina
- Language:Chinese
-
Abstract:
Objective To expore multi-drug resistant Acinetobacter baumannii efflux pump phenotype and efflux pump gene expression in the resistant isolates. Methods Application of K-B method to detect 96 strains isolated from the First Hospital of Hebei Medical University multi-drug resistant Acinetobacter baumannii′ resistance to 15 kinds of antibacterial drugs, detecting multi-drug resistant Acinetobacter baumannii efflux pump phenotype with broth microdilution method by the addition of carbonyl cyanide chlorobenzene hydrazone ( CCCP) pump inhibitors,using PCR amplification and sequencing to study efflux pump protein gene sequence characteristics . Results The Acinetobacter baumannii resistance rate of 96 strains to quinolones, cephalosporins, aminoglycosides, tetracyclines were 70. 8%-94. 8%.There were 34 positive efflux pump phenotypes in 96 multi-drug resistant Acinetobacter baumannii strains, including 33 adeB strains, 32 adeR strains, 33 adeS strains, 33 adeJ strains,0 adeE strain,33 adeM strains, positive detection rate were 97. 06%, 94. 12%, 97. 06%, 97. 06%, 0, 97. 06%, respectively. By sequence comparison, adeB, adeR and adeS genes sequence homology was 100% in the GenBank. Conclusion Active efflux pump gene perturbation is one of the important factors in multi-drug resistant Acinetobacter baumannii.
