Expression and significance of NGAL in MRL/lpr lupus-prone mice
10.3760/cma.j.issn.1007-7480.2014.10.009
- VernacularTitle:中性粒细胞明胶酶相关脂质运载蛋白在狼疮肾炎小鼠模型中的表达及其意义
- Author:
Hua ZHANG
;
Liang XU
;
Huitao ZHANG
;
Jing ZHENG
;
Ning JIA
;
Ye ZHU
;
Lingling LI
;
Yujing LIN
;
Zhonghe LI
- Publication Type:Journal Article
- Keywords:
Lupus nephritis;
Interleukin-17;
Gelatinase B;
Neutrophil gelatinase-associated lipocalin;
Tissue inhibitor of metalloproteinase-1
- From:
Chinese Journal of Rheumatology
2014;(10):692-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression and significance of neutrophil gelatinase-associated lipocalin (NGAL) in lupus nephritis (LN) in mice. Methods Female MRL/lpr lupus mice (n=36) were randomly divided into the experimental group and intervention group, and female Kunming mice (n =18) served as controls. Each mice in the intervention group received intraperitoneal injection of 20 μg anti-mice interleukin (IL)-17 antibody. The serum concentrations of NGAL, IL-17,matrix metalloproteinase(MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 were measured by enzyme-linked immunosorbent assay (ELISA). And the protein expressions of NGAL, IL-17, MMP-9 and TIMP-1 in renal tissue were detected by immunohistochemisty. One-factor analysis of variance (ANOVA) or nonparametric rank sum test was used for the comparisons between the three groups. Associations between these factors were analyzed by Pearson′s test. Results The levels of serum NGAL, IL-17, MMP-9 and TIMP-1 in the experimental group were obviously increased as compared to those in the control group and intervention group [NGAL: (30.31±1.22) ng/ml vs (11.36±0.14) ng/ml, (20.09±0.35) ng/ml, F=986.524, P<0.001]. The protein expression of NGAL, IL-17, MMP-9 and TIMP-1 in the renal tubular epithelial cells in the experimental group was increased as compared to the control group and intervention group[NGAL:(11.27±0.58) vs(0.45±0.19),(9.22±0.67), H=15.158, P =0.001]. In the experimental group, a positive correlation was found between the level of serum NGAL
and the serum levels of IL-17, MMP-9 and MMP-9/TIMP-1(r=0.899, 0.789, 0.925, P<0.01). The protein expression of NGAL in renal tissue was positively correlated with IL-17, MMP-9 and MMP-9/TIMP-1 levels (r=0.929, 0.899, 0.723, P<0.01). Conclusion The level of NGAL in the serum and renal tissue is signifi-cantly increased in the MRL/lpr lupus mice. And it is closely correlated with the levels of IL-17 and MMP-9. Our results suggest the potential role of NGAL in the inflammation of lupus nephritis.
