Identification of Major Metabolites of Salbutamol in Swine Urine and Plasma Using Ultra-High Performance Liquid Chromatography-Electrospray-Time of Flight-Mass Spectrometry
10.11895/j.issn.0253-3820.140356
- VernacularTitle:超高压液相色谱-飞行时间质谱法对比分析沙丁胺醇在猪尿液和血浆中的代谢产物
- Author:
Xu GU
;
Yiming LIU
;
Ting YAO
;
Huale SHI
;
Jun LI
;
Zhen ZHAO
;
Yuchang QIN
- Publication Type:Journal Article
- Keywords:
Salbutamol;
Ultra-high performance liquid chromatography-electrospray-time of flight-mass spectrometry;
Metabolites
- From:
Chinese Journal of Analytical Chemistry
2014;(11):1692-1696
- CountryChina
- Language:Chinese
-
Abstract:
Theexcretionofurinaryandplasmametabolitesofsalbutamolwasstudiedusingultrahigh performance liquid chromatography electrospray time-of-flight mass spectrometry, after a single intragastric gavaged dose administration with salbutamol. The software of Agilent MassHunter Metabolite ID was employed to find and identify the chemical structure of metabolites of salbutamol. Five metabolites from salbutamol were identified. Metabolites identified in swine urine included glucuronide conjugate salbutamol, N-oxide-salbutamol, hydroxyl-salbutamol, methoxyl-salbutamol and dehydrated hydroxyl-salbutamol. Whereas, only the parent drug, glucuronide conjugate salbutamol and dehydrated hydroxyl-salbutamol were observed in swine plasma.
