Study on Methodolgy of Content Determination of Three Flavonoids in Traditional She Medicine Shi Liang Cha
- VernacularTitle:畲药食凉茶中三种黄酮类成分含量测定的方法学研究
- Author:
Juhua MAO
;
Weiying WANG
;
Le YU
;
Kejun CHENG
- Publication Type:Journal Article
- Keywords:
Traditional She medicine;
Shi Liang Cha;
Rutin;
Quercetin;
Kaempferol;
HPLC
- From:
China Pharmacist
2014;(11):1808-1810
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for the simultaneous determination of three flavonoids in Shi Liang Cha. Methods:HPLC was applied with the chromatographic conditions as follows: the chromatographic column was Agilent Zorbax SB-C18 (250 mm × 4. 6 mm, 5μm) at 30℃;acetonitrile-0. 1% H3 PO4 solution was used as the mobile phase with gradient elution; the flow rate was 1. 0 ml·min-1, and the detection wavelength was 360nm. Results: Good linearity of rutin, quercetin and kaempferol was within the range of 0.0409-1.637 0mg·ml-1(r=0.999 2), 0.44-88.00μg·ml-1(r=0.999 8) and 0.41-77.63μg·ml-1(r=0. 999 2), respectively; the average recovery was 99.35%(RSD =1. 64%),101. 14% (RSD =1. 88%) and 99. 69% (RSD =1. 92%) , respectively. Conclusion:The method is simple, accurate and repeatable, and suitable for the simultaneous determination of rutin, quercetin and kaempferol in Shi Liang Cha.
