Determination of Tetracyclic Triterpene Saponins in Sanqi Jiegu Pills and Stability of Saponins in Granula-tion
- VernacularTitle:三七接骨丸中四环三萜类皂苷的含量测定及制粒稳定性研究
- Author:
Jialin WANG
;
Fang CHENG
;
Jing ZHAO
;
Runqin GUO
- Publication Type:Journal Article
- Keywords:
Sanqi Jiegu pills;
HPLC;
Gensenoside Rg1;
Gensenoside Rb1;
Notogensenoside R1
- From:
China Pharmacist
2014;(11):1873-1876
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination gensenoside Rg1 , Rb1 and notogensenoside R1 in Sanqi Jiegu pills and to investigate the stability of saponins in the granulation process. Methods:The separation was performes on a COSMO-SIL 5 C18 MS-II(250 mm × 4. 6 mm,5μm)column with gradient elution using water and acetonitrile as the mobile phase, the flow rate was 1. 0 ml·min-1 , the detection wavelength was 203nm, the column temperature was 30℃, and the injection volume was 10 μl. The content change of triterpene saponins in Sanqi Jiegu pills prepared by the granulation process at different drying temperature was al-so investigated. Results:The linearity of notogenoside R1 , gensenoside Rg1 and gensenoside Rb1 was good within the range of 0. 211 8-2.648 0 μg·ml-1(r=0.999 7),0.566 1-7.076 0 μg·ml-1(r=0.999 7) and 0.317 2-3.964 5μg·ml-1(r=0.999 7) with the average recovery of 98. 2%(RSD=0. 82%,n=6), 98. 1%(RSD=0. 72%,n=6) and 98. 1%(RSD=0. 59%,n=6),respectively. The content of saponins in Saqi Jiegu pills was decreased obviously with the increase of the drying temperature. Conclusion:The meth-od is simple, quick and reliable, and can be applied in the quality control of Sanqi Jiegu pills. It is recommended that the drying tem-perature in the granulation process should be controlled below 60℃.
