Regulatory effects of CD44 antibody-A3 D8 on IL-3 Rαand downstream PI3 K/Akt pathway in NB4 cells

VernacularTitle:CD44单抗A3 D8对NB4细胞IL-3 Rα及其下游信号通路的调节
Author: Ping CHEN ; Qin YUAN ; Xi JIANG ; Juanying WU ; Huifang HUANG
Publication Type:Journal Article
Keywords: CD44; A3 D8; NB4; IL-3 Rα; PI3 K/Akt pathway; LY294002
From: Chinese Pharmacological Bulletin 2014;(11):1526-1529
CountryChina
Language:Chinese
Abstract: Aim To investigate the effect of CD44 anti-body-A3 D8 on the expression of IL-3 Rα and down-stream PI3K/Akt in NB4 cells. Methods The ex-pression of IL-3 Rα mRNA was detected by real-time quantitative RT-PCR, the IL-3Rα protein expression and changes of PI3 K/Akt signal pathway in NB4 cells treated with A3D8 were analyzed by Western blot. An-nexin-V-FITC/PI double staining flow cytometry was u-tilized to detect the apoptotic cells. The inhibitor of PI3 K/Akt signaling LY294002 combined with A3 D8 was used to inhibit the PI3K/Akt in NB4 cells. Re-sults After treated with A3 D8 , both the transcription-al level and translational level of IL-3 Rα were remark-ably reduced, and the PI3K/Akt pathway was inhibi-ted. LY294002 improved the inhibitory and apoptotic effects of A3D8 on NB4 cells. Conclusion CD44 antibody A3 D8 can downregulate the expression of IL-3Rα and inhibit the downstream PI3K/Akt pathway.