Determination of Ursolic Acid in Zhike Chuanbei Pipa Syrups by HPLC
- VernacularTitle:高效液相色谱法测定治咳川贝枇杷露中熊果酸的含量
- Author:
Fang QIAN
;
Feifan XUE
- Publication Type:Journal Article
- Keywords:
Zhike Chuanbei Pipa syrups;
Ursolic acid;
HPLC;
Determination
- From:
China Pharmacist
2014;(11):1975-1976
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to determine the content of ursolic acid in Zhike Chuanbei Pipa syrups. Methods:An HPLC method was used with a Cosmosil-C18 column(250 mm × 4. 6 mm, 5 μm). The mobile phase was methanol-water-glacial acetic acid-triethylamine(90∶10∶0. 03∶0. 06) with a flow rate of 1. 0 ml·min-1 , the column temperature was 25℃, and the detec-tion wavelength was set at 215nm. Results:The linear range of ursolic acid was 16-80μg·ml-1(r=0. 999 5, n=5), and the aver-age recovery was 98. 67℅ (RSD=1. 22, n=6). Conclusion:The method is simple, accurate and reliable with good specificity and reproducibility, and it can be used in the quantitative determination of ursolic acid in Zhike Chuanbei Pipa syrups.
