TNF-αenhances IFN-γ-induced NIT-1 cells apoptosis via death receptor pathway
10.3969/j.issn.1000-484X.2014.11.002
- VernacularTitle:TNF-α通过死亡受体途径促进IFN-γ诱导NIT-1细胞凋亡
- Author:
Shifang DONG
;
Guiqing LI
;
Weifan JIANG
;
Fei YANG
;
Zhaohui CAO
;
Guilian XU
- Publication Type:Journal Article
- Keywords:
TNF-α;
IFN-γ;
Islet-cells;
T1DM
- From:
Chinese Journal of Immunology
2014;(11):1448-1451
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of TNF-αand IFN-γon NIT-1 cells apoptosis and the apoptosis mechan-ism.Methods:NIT-1 cells were exposed to a combination of TNF-αand IFN-γtreatment.The viability of NIT-1 cells was assessed via MTT assay.The morphological changes of the cells and nuclei were observed under the inverted or confocal laser scanning microscope with Hoechst 33258 staining.The activation of Caspase-8,-3 and PARP was detected by Western blot.Results:TNF-αand IFN-γsig-nificantly inhibited NIT-1 cells viability, promoted cells apoptosis, induced the activation of Caspase-8,-3 and PARP cleavage.Conclusion:TNF-αcombined with IFN-γtreatment induced the apoptosis of NIT-1 cells through death receptor pathway.
