Application of HLA-A*0201/CAP-1 tetramer in colorectal cancer
10.3969/j.issn.1000-484X.2014.11.012
- VernacularTitle:HLA-A*0201／CAP-1四聚体在结直肠癌研究中的应用
- Author:
Yayun QIAN
;
Mingchun JI
;
Yanqing LIU
;
Weijuan GONG
- Publication Type:Journal Article
- Keywords:
MHC class Ⅰmolecules tetramers;
Cytotoxic T lymphocytes;
Colorectal cancer;
Carcinoembryonic antigen
- From:
Chinese Journal of Immunology
2014;(11):1494-1498
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the frequencies of HLA-A*0201 restricted CEA-specific CD8+T cells, HLA-A*0201/FLUmp tetramer and HLA-A*0201/CAP-1 tetramer were applied in patients with colorectal cancer.Methods: Lymphocytes from peripheral blood and lymph node,1×106 cells/ml,were incubated with 1μg HLA-A*0201/peptide tetramers and anti-CD8 for 1 h at 25 coseperately.The cells were then washed in PBS.Next,the cells were illuminated by detecting frequencies of FLUmp-specific CD8+T cells and CAP-1-specific CD8+T cells with flow cytometry.Results: HLA-A*0201/peptide were used to detect CAP-1 or FLUmp-specific CD8+T cells,which were analyzed either healthy individuals or patients with colorectal cancer.We did not find differences in average frequencies of FLUmp-specific CD8+T cells between 11 HLA-A*0201+patients with colorectal cancer and 14 HLA-A*0201+healthy individuals [ ( 0.671 ±0.421 )%, ( 0.564 ±0.408 )%].But the frequencies of CAP-1-specific CD8+T cells of HLA-A*0201+patients with colorectal cancer showed higher than HLA-A*0201+healthy individuals [ ( 2.409 ± 2.385 )%, ( 0.020 ± 0.021)%respectively],which was statistically significant(P=0.008).Conclusion:The frequencies of CAP-1-specific CD8+T cells in PBMC from peripheral blood and lymph node of HLA-A*0201+patients were increased,showed CEA-specific CTs has a vital role in colorectal cancer.
