Melanoma antigen A3 sourced from laryngeal cancer was expressed in mouse melanoma cell model
10.3969/j.issn.1000-484X.2014.11.017
- VernacularTitle:喉癌来源黑色素瘤抗原A3在小鼠黑色素瘤细胞模型的表达
- Author:
Ning LI
;
Xiaobin JI
;
Jinghua XIE
;
Qicai LIU
- Publication Type:Journal Article
- Keywords:
Melanoma-associated antigen A3;
Eukaryotic expression vector;
Gene transfection;
Green fluorescent protein;
Expression;
The tumor cells model
- From:
Chinese Journal of Immunology
2014;(11):1517-1522
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct tumor cell model by determination of the pIRES2-EGFP/MAGE-A3 eukaryotic expression plasmid expressing steadily in mouse melanoma B16 cells.Methods:The pIRES2-EGFP/MAGE-A3 eukaryotic expression plasmid being constructed from the melanoma-associated antigen A3 genes sourcing laryngocarcinoma in advance was translated into the mouse melanoma B16 cells under the mediation of lipofectamine,and the positive clones were detected with G418.The expression of enhanced green fluorescent protein( EGFP) and MAGE-A3 mRNA in positive clones were detected by fluorescence microscopy and fluorescence quantitative PCR ( qRT-PCR ) assay, respectively.Results:The pIRES2-EGFP/MAGE-A3 eukaryotic expression plasmid has been transfected into B16 cells successfully, the green fluorescence of fusion protein expression was found, and MAGE-A3 mRNA transcription in B16 cells expressions were detected in positive clones.Conclusion:The pIRES2-EGFP/MAGE-A3 eukaryotic expression plasmid has been transfected effectively and expressed stably by liposome method in the B16 cells.The expression of MAGE-A3 tumor cell model has been successfully established,which provide data for the study of laryngocarcinoma immunotherapy.
