Analysis of effects of allicin prodrug on proliferation of esophageal cancer cell line Eca9706 and the expression of apoptosis gene
- VernacularTitle:大蒜素前药对食管癌细胞Eca9706增殖及凋亡基因表达的影响分析
- Author:
Yongdong CHEN
;
Zhongfu SHAO
- Publication Type:Journal Article
- Keywords:
allicin prodrug;
esophageal cancer cell line Eca9706;
proliferation;
apoptosis gene
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;37(7):43-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore and analyze the effects of allicin prodrug on proliferation of esophageal cancer cell line Eca9706 and the expression of apoptosis gene.Methods Different concentrations of allicin prodrugs were divided into a total of four groups:A1 group(10μg/mL),A2 group(20 μg/mL),A3 group (40 μg/mL),A4 group (normal saline,0 μg/mL),and respectively applicated in esophageal carcinoma cell line Eca9706.After culturing for 1 days,2 days,3 days,cell proliferation was detected by MTT and expression of p53 at the mRNA level was detected by RT-PCR.Results The optimum concentration of proliferation inhibition of allicin prodrug on esophageal cancer cell line Eca9706 was 20μg/mL and the best inhibition time was 2 days;the gene level of esophageal cancer cell line Eca9706 was inhibited with a dose-dependent.Conclusion Allicin prodrugs could effectively inhibit the proliferation of esophageal cancer cell line Eca9706,and control the proliferation of esophageal cancer cells by regulating the expression of apoptosis associated genes,so as to eliminate tumor cells.
